Eppendorf BioSpectrometer kinetic (Demo)

€4.999,00 * Excl. tax
Article number 5500-203
Availability: In stock (5)

Eppendorf BioSpectrometer kinetic

Demo instrument. Excellent condition - great price. Comes in original packaging with 12 months manufacturer's warranty.

 

 Features

  • Absorbance measurement for one or more wavelengths, recording of wavelength scans from 200 nm to 830 nm (increment: 1 nm)
  • Automatic evaluation and storage of measurement data with clear presentation of results
  • Spectral graph to display sample purity with automatic ratio calculation
  • Preprogrammed applications for quick start
  • Freely programmable applications with evaluation via factor, standard or standard series and freely programmable parameters
  • Two-wavelength method with substraction or division evaluation
  • Integrated application and results memory
  • Data transfer via USB interface, Ethernet or via e-mail and direct printout of results
  • Guided software process to minimize errors
  • Integrated self-test and calibration history
  • Temperature-controlled cuvette shaft (adjustment range +20 °C to +42 °C in 0.1 °C increments)
  • Preprogrammed and freely programmable kinetic methods
  • Retrospective modification of the time window for regression analysis

 

Applications

  • Nucleic acid quantification
  • Direct protein quantification (UV 280 nm)
  • Microvolume measurement via µCuvette G1.0 for highly concentrated samples
  • Bacteria growth measurement (OD 600)
  • Colorimetric assays for protein quantification, e.g., BCA, Bradford, Lowry
  • Evaluation of FOI for dye-labeled biomolecules (nucleic acid or proteins)
  • Free selectable wavelengths, e.g., 340 nm: assays using NADPH or NAPH, 405 nm: assays using para-nitrophenol, 420 nm: assays using ortho-nitrophenol, 490 nm: colorimetric fructose determination, 490 nm: cytotoxicity assay
  • kinetic applications, and enzyme activity testings at different temperatures e.g.:
    • Activation of glucose via hexokinase (340 nm, 37 °C)
    • Substrate concentration of ATP (340 nm, 37 °C)
    • Enzymatic assay of β-galactosidase (420 nm, 30 °C)

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