Marker
| G100 Ladder |
| Description |
DNA 100 bp Ladder |
| Appearance on Gel |
10 discrete fragments:
100, 200, 300, 400, 500 (2x), 600, 700, 800, 900, 1000.
The bright band at 500 bp serves as a reference. |
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|
| Storage Buffer |
10 mM Tris-HCl (pH 7.6), 1 mM EDTA |
| Concentration |
0.25 µg/µl |
| Order Number / Quantity |
NM100 / 50 µl 40,- Euro |
| Storage Condition |
Store at -20°C |
|
| G200 Ladder |
| Description |
DNA 200 bp Ladder |
| Appearance on Gel |
Fragment Sizes in Base-Pairs
6 discrete fragments:
2000, 1000, 800, 600, 400, 200 |
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|
| Storage Buffer |
10 mM Tris-HCl (pH 7.6), 1 mM EDTA |
| Concentration |
0.25 µg/µl |
| Order Number / Quantity |
NM200 / 25 µg 45,- Euro |
| Storage Condition |
Store at -20°C |
|
| G1K |
| Description |
DNA 1 kp Ladder |
| Appearance on Gel |
Fragment Sizes in Base-Pairs
13 discrete fragments:
250, 500, 750, 1000 (3x), 1500, 2000,
2500, 3000 (3x), 4000, 5000, 6000, 8000, 10000
The bright bands at 1000 bp and 3000 bp serve as a reference. |
 |
|
| Storage Buffer |
10 mM Tris-HCl (pH 7.6), 1 mM EDTA |
| Concentration |
0.25 µg/µl |
| Order Number / Quantity |
NM1k / 250 µg 75,- Euro |
| Storage Condition |
Store at -20°C |
|
| pUC19-HpaII Marker |
| Description |
DNA Marker for short fragments. pUC19 DNA was completely digested with HpaII (MspI), phenol extracted, ethanol precipitated and dissolved in TE. |
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Note: The 34 & 26 bp fragments are only visible on overloaded gels. Fragmets in brackets appear as one band. |
| Storage Buffer |
10 mM Tris-HCl (pH 7.6), 1 mM EDTA |
| Concentration |
0.5 µg/µl |
| Order Number / Quantity |
NM141 / 50 µg 40,- Euro |
| Storage Condition |
Store at -20°C |
|
| Lambda DNA BstEII Marker |
| Description |
DNA Marker: Lambda DNA was completely digested with BstEII and EcoRI, phenol extracted, ethanol precipitated and dissolved in TE buffer (Storage Buffer) |
| Appearance on Gel |
Fragment Sizes in Base-Pairs
14 discrete fragments (in base pairs): 8453*, 7242, 6369, 5687*, 4822, 4324, 3675, 2323, 1929, 1371, 1264, 702, 224, 117.
* The cohesive ends (12b cos site of bacteriophage Lambda) of fragments 8453 bp and 5678 bp may anneal and form an additional band. Fragments marked by "*" can be separated by heating to 65°C for 5 minutes and cooling on ice for 3 min. |
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|
| Storage Buffer |
10 mM Tris-HCl (pH 7.6), 1 mM EDTA |
| Concentration |
0.3-0.5 µg/µl |
| Order Number / Quantity |
NM108 / 250 µg 35,- Euro |
| Storage Condition |
Store at -20°C |
|
| Lambda DNA EcoRI/HindIII Marker |
| Description |
DNA Marker: Lambda DNA was completely digested with HindIII and EcoRI, phenol extracted, ethanol precipitated and dissolved in TE buffer (Storage Buffer) |
| Appearance on Gel |
Fragment Sizes in Base-Pairs
12 discrete fragments (in base pairs): 21226*, 5148, 4973, 4268, 3530*, 2027, 1904, 1584, 1375, 947, 831, 564, 125.
* The cohesive ends (12b cos site of bacteriophage Lambda) of fragments 21226 bp and 3530 bp may anneal and form an additional band. Fragments marked by "*" can be separated by heating to 65°C for 5 minutes and cooling on ice for 3 min. |
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|
| Storage Buffer |
10 mM Tris-HCl (pH 7.6), 1 mM EDTA |
| Concentration |
0.3-0.5 µg/µl |
| Order Number / Quantity |
NM102 / 250 µg 35,- Euro |
| Storage Condition |
Store at -20°C |
|
| Lambda DNA EcoRI Marker |
| Description |
DNA Marker: Lambda DNA was completely digested with EcoRI, phenol extracted, ethanol precipitated and dissolved in TE buffer (Storage Buffer) |
| Appearance on Gel |
Fragment Sizes in Base-Pairs
6 discrete fragments (in base pairs): 21226*, 7421, 5804, 5643, 4878, 3530*
* The cohesive ends (12b cos site of bacteriophage Lambda) of fragments 21226 bp and 3530 bp may anneal and form an additional band. Fragments marked by "*" can be separated by heating to 65°C for 5 minutes and cooling on ice for 3 min. |
 |
|
| Storage Buffer |
10 mM Tris-HCl (pH 7.6), 1 mM EDTA |
| Concentration |
0.3-0.5 µg/µl |
| Order Number / Quantity |
NM110 / 250 µg 35,- Euro |
| Storage Condition |
Store at -20°C |
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